jamie finally finished his presentation, before which he was so nervous. but he didn't show his anxiety in front of us. some ppl are just like him, appearing very calm right before exams or presentations but that doesn't mean they don't care about things they gonna do. he's expecting to complete his PhD in one year i think, cos i remember someone told me if one gets to do a presentation in front of department profs in a seminar, that means he's gonna get his degree soon if everything's going fine. pulleyblank the you-knew-all was absent today, and this made jamie a little bit happier i guess. since pulleyblank always asks very very smart questions. jamie joked right before the presentation that he wished that pulleyblank could tell him what RavA does. moran asked some questions in and after the presentation and my prof was thinking moran's really annoying, cos he kept on asking about bioinformatics which are not thoroughly researched due to the nature of the project. my prof was like, "i really want to beat him up to stop him from asking so many irrelevant questions." that's funny. i'm still quite scared of houry, cos everytime i see him i just feel so quilty about me not getting the life sci award, so that he got to pay me from his funding. i was unhappy about his reluctancy before, but now i just feel that i should have done better to avoid this problem. anyway, what i can do now is to work hard to reciprocate.
asad failed his G2 test, i think it's normal. wash failed his G1 three times. i can't believe it. stupid guy. asad got into med school and everyone's congratulating him, so G2 test failure wasn't gonna matter anymore. he was still happy and chipper.
ok, here comes what i did today.
- poured three agarose gels... almost became an expert now.
- did RE digestion of plasmids. RE must be kept on ice and added the latest.
- ran gels with keith. the tip should be strait into the well and hands steady during gel loading.
- get a cool picture of my gel, finally grasped how to take gel pics using the machine.
- helped asad with BamHI digestion. ran a gel but didn't get good results. apparently the plasmids weren't cut. they still ran as one whole band.
- learnt to make LB media. this's recipe: 1 litre of distilled distilled water (DDS), 5g of yeast extracts, 10g of salts, 10g of casein(?) amino acid nutrients. mix these up but ensure the volume of water should be 1 litre. i.e. take out some water before adding the threesome. then add back some water to make up to 1 litre after the threesome's added.
- autoclaved LB in flasks with autoclaving tapes
i made some really stupid mistakes when following keith. the gloves on my hands were too big, so when i tried to close the microcentrifuge tubes the gloves always got stuck under the cap. and i accidentally slipped one tube away from my hand. lucky the tube's tightly closed. but keith was like, never mind, it's just a diagnostic one. and he actually planned to re-do one round later. i felt very embarrassed and ashamed. i could do better actually. when it came to gel loading, which is my favourite cos i consider myself good at it, i still sort of screwed it. or, i should say, keith's too good at it. anyway i don't want to find an excuse for myself. but lucky lucky the gel turned out to be quite good, and apparently keith was quite excited about it. haha. it's not that bad.
so my homework's reading cloning paper... jamie's back to normal, that means he's gonna be my real boss from tmr onwards and he's gonna make me do real stuff soon enough. so my suffering has just started. gonna skip the stupid house meeting and read the primary paper later.
did a funny test:
| You Belong in Dublin |
 Friendly and down to earth, you want to enjoy Europe without snobbery or pretensions. You're the perfect person to go wild on a pub crawl... or enjoy a quiet bike ride through the old part of town. |
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